Sexual violence occurs in every culture, in all levels of society, and in every country of the world. In cases of sexual assault, especially in rape, examinations often involve searching items such as clothing and bedding for seminal stains.12 The seminal stains recovered on fabrics in the form of stained clothing, undergarments, and swabs on fabric material taken from the vagina and surrounding regions are submitted to DNA fingerprinting tests to compare them with the blood sample of the accused person. Isolation of DNA from such stains, particularly when the stains are old, is usually not possible. The causes could be degradation or decomposition of the biological material,which depends mainly upon the environmental conditions. Extraction was performed by using a genomic DNA purification kit (QIA amp DNA investigator kit for isolation of total genomic DNA from material stained with semen) on the first day as well as after 14 and 30 days. The results from the first day were used as reference samples. DNA quantity in dried seminal stains decreases with increased storage duration, and DNA recovery from natural fabrics (cotton and silk) is much higher than from artificial fabric (polyester), with the maximum yield from cotton followed by silk and polyester. Therefore, in the medicolegal practice, it is recommended that cotton fabric be used for swabs for collection of seminal stains.
This article affects all of humanity because rape is a serious problem around the whole world. With the help of DNA being extracted from clothing, many victims can be helped by the catching of the perpetrator.
The article was long and structured. It broke down everything that was needed to be known and was well written. All key points were addressed and everything was explained properly.
No comments:
Post a Comment